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DEPRECATED- This is in Seattle- we use off-site sequencing for all sequenceing now at the Buck; see Sequencing with Operon for details.


The UW DNA Sequencing Facility is in Hitchcock 223. On your first use of the facility, you'll need to create a login, including funding information, which you'll have to ask someone in the lab for (if in doubt ask Soumya).


The website here explains how to turn in your samples, which depends on what kind of DNA you're sending but basically: you place your order online, print out a form from their website onto colored paper, put your template and primers into 1.5 ml Eppendorf tubes at the appropriate concentrations, and put your tubes in the tube box on the top shelf of the 4C just inside the door of Hitchcock 223, and your printed out form (on colored paper) into the labeled box for the forms. Then a day or two later you'll get an email with a link to download your zipped results.


They want our "Box for Billing" on the order form; it's our campus box, 357350.


These results are confusingly named by the default output of the sequencer; if you want them named like the original numbered sample names that you submitted, I wrote a Perl script to do that, come and ask me.


Or, here:

#!/usr/bin/perl -w
use strict;
 
# sequencingrenamer.pl 04-13-10 last modified 04-13-10
# Our University of Washington sequencing facility asks for your sequencing names as your initials plus a number;
# you can never re-use numbers for a user account so your lifetime sequencing submissions will read as MM1, MM2,  
# or whatever.  They then return output back to you with names from the sequencer's direct
# output, which contain somehwere within the long name your actual input sequencing run name, e.g.
# H08_MM12_Plate7222_050.seq or H08_MM12_Plate7222_050.ab1, for MM12.  These are then non-optimal to sort or work with
# .  This script, when run in a directory of these sequencer-named files,  
# gives back the same files with the names you'd want e.g. MM12.seq or MM12.ab1.  This version is hard coded to my own
# initials, MM.  Edit it for yours.  
 
my $globstring = "*MM*.*";
my @files = glob "$globstring";
 
foreach my $files(@files) {
    if ($files =~ /.*(MM\d+).*\.(.*)/) {
        my $newname = "$1.$2";
        rename($files, $newname);
    }
}
 
# end for now, working 04-13-10