For bacterial strains, the same basic protocol can be used as with yeast strains; by starting with 990ul from an LB or LB + selection antibiotic (e.g. Amp) overnight culture plus 330ul 80% Glycerol Stock, except that with bacterial strains it's recommended to snap freeze in either Liquid Nitrogen or a Dry Ice ethanol bath before putting them in the -80C freezer.

Glycerol: (Sigma-Aldrich, G7893-2L)