Getting Started With Yeast is a how-to written by Fred Sherman at the University of Rochester School of Medicine and Dentistry.

You can download a pdf of it here or maybe here.

If you're going to be working with yeast, read through this when you have time, and ask anyone in lab who works with yeast if you have further questions or want more info.

The protocols here may differ from the exact way we do things, but will still give you the general outline.

Note that this guide is from 2002, so developments in techniques since then won't be reflected.