Our qPCR system is an iCycler iQ qPCR system from Bio-Rad, it's in the Merz lab, in HSB J312. The manual for the base iCycler is here, and for the iQ qPCR upgrade is here. There are also specific application notes for general protocol, melt curves, PCR quantification, and allelic discrimination online. The primer for doing qPCR is the Bio-Rad Real-Time PCR Applications Guide, 105pp and $21.00 from Bio-Rad bound, but also available as a pdf here.

Vivian has prepared a set of ppt slides outlining our lab specific best practices for RNA work including PCR; ask anyone in the lab (including Vivian obvs) to get a copy by email.