Our Plate Camera for taking pictures of yeast plates is in the rear section of J330, aka J330A, aka the scope room.
It has two lights, you should turn them both on with their individual switches and be sure to turn them off when you're done.
Use the H2O squirt bottle nearby to add a puddle of water to the black background, so that it fills in the space betweeen your plate and the background when you set the pate down. Be sure to clean up your plates and the area after you're done.At the buck, use room "4 293 Dark Room" to take your pictures. It is across the hall from our lab. Please do not leave the camera or a mess in this space.
The camera is pretty self-explanatory, if in any doubt ask someone to show you.
When you're done eject the large memory card from the left side of the camera (again ASK if it isn't plain how to eject it), and use the eFilm USB Adapter in the box on the shelf directly above the camera to transfer the pictures to your comupter right away, and return the USB adapter and replace the memory card right away. Note: the CF card used in our camera is unkeyed; it can be inserted in two different orientations, only one of which works. When removing it, look at the labels on the card and reinsert it in the same orientation. similarly, if the eFilm adapter isn't recognized by your computer, chances are you've got the CF card in it backwards so flip it over.
Always print hard copies of your plate pictures for your lab notebook in addition to saving them on your computer. You can add text labels directly to the pictures before printing easily using Photoshop or GiMP. In GiMP, you can often greatly improve the visibility of your tetrad replica colonies or transformation colonies by using Auto Levels: after opening the image, go to Colors>Levels and select Auto. You can cancel or undo if you don't like the result.