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Once upon a time we found a male nematode. We wanted to propagate a male plate.

To propagate we want to take the male that we found and move him to a small plate.

We need to find a L3/L4 Hermaphrodite and place him/her on the same plate with our male friend.

Next place a small glob of OP-50 from another plate (about the size of a pencil eraser) to keep them in close proximity to each other.

Place them back into the incubator and check up on them 2-3 days later

(Optional: play Berry White and lower the light level)

When you get back to them they should be half male and half hermaphrodite.

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